Dinucleotide Junction Cleavage Versatility of 8-17 Deoxyribozyme
نویسندگان
چکیده
منابع مشابه
Sequence-function relationships provide new insight into the cleavage site selectivity of the 8–17 RNA-cleaving deoxyribozyme
Many sequence variations of the 8-17 RNA-cleaving deoxyribozyme have been isolated through in vitro selection. In an effort to understand how these sequence variations affect cleavage site selectivity, we systematically mutated the catalytic core of 8-17 and measured the cleavage activity of each mutant deoxyribozyme against all 16 possible chimeric (RNA/DNA) dinucleotide junctions. We observed...
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ژورنال
عنوان ژورنال: Chemistry & Biology
سال: 2004
ISSN: 1074-5521
DOI: 10.1016/j.chembiol.2003.12.012